《植物生理学报》 2011, 47(2): 141-146

植物基因工程中人工启动子的研究进展

彭舒^1,2, 黄真池¹, 欧阳乐军^1,2, 程杰^1,2, 曾富华^1,*

1湛江师范学院 生命科学与技术学院, 广东湛江524048; 2湖南农业大学生物科学技术学院, 长沙410128

通信作者：曾富华；E-mail: zengfuhua@gmail.com；Tel: 0759-3183622

摘　要：

启动子是调控基因转录的一段DNA, 也是构建基因工程表达载体的重要元件。天然启动子在表达强度和特异性等方 面存在一定的局限性。采用人工构建的方法, 有望得到诱导因子广、本底活性低、表达强度高、启动表达快等特点的启 动子。本文综述了人工启动子在诱导表达、组织特异性表达、高效表达等方面的研究进展。

关键词： zengfuhua@gmail.com

收稿：2010-10-25   修定：2010-12-29

资助：广东省自然科学基金(8152404801000011、10152404801000005、10452404801004328)。

Research Progress of Artificial Promoter in Plant Genetic Engineering

PENG Shu^1,2, HUANG Zhen-Chi¹, OUYANG Le-Jun^1,2, CHENG Jie^1,2, ZENG Fu-Hua^1,*

¹School of Life Science & Technology, Zhanjiang Normal University, Zhanjiang, Guangdong 524048, China; ²College of Bioscience and Biotechnology, Hunan Agriculture University, Changsha 410128, China

Corresponding author: ZENG Fu-Hua; E-mail: zengfuhua@gmail.com; Tel: 0759-3183622

Abstract:

Promoter is a piece of DNA fragment that regulates gene transcription by allowing RNA polymerase and transcription factors binding to it. Promoter is also an important element in the expression vectors in genetic engineering. However, there are some limitations in natural promoter, such as low expression activity and low specificity. Alternatively, artificial constructed promoters are expected to overcome those limits and achieve advantages, such as more induction factors, low basal activity, high expression activity and quick expression initiation. In this paper, we reviewed research advances in artificial promoter constructions in terms of the inducible expression, tissue-specific expression and high-efficiency expression.

Key words: artificial promoter; inducible expression; tissue-specific expression; high-efficiency expression